Enzymes
Phosphorylation
Nucleoside kinases
Thymidine kinase NK02
Art.-No.: E-KI-0102
Bacterial origin, thermostable up to 80°C
160,00 €
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The thymidine kinase NK02 is a thermostable enzyme of bacterial origin. It phosphorylates thymidine and its derivatives to the respective NMPs using ATP as phosphate donor. Deoxycytidine, deoxyguanosine, deoxyinosine, uridine and deoxyuridine can also act as acceptor, dGTP can act as a donor. The quasi-irreversible transfer of phosphate from the high energy donor to the nucleoside enables high NMP yields.
EC Number
2.7.1.21
CAS Number
9002-06-6
Catalyzed reaction(s)
ATP + thymidine = ADP + dTMP
Synonyms
TK, 2'-deoxythymidine kinase, ATP:thymidine 5'-phosphotransferase
Origin
Bacterial
Molecular weight
22.1 kDa
Other characteristics
Thermostable up to 80°C
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Deoxynucleoside kinase NK12
Art.-No.: E-KI-0112
Drosophila melanogaster dnk, mesophilic
180,00 €
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The deoxynucleoside kinase NK12 is a mesophilic enzyme originating from Drosophila melanogaster. It phosphorylates deoxynucleosides to dNMPs using (d)ATP as phosphate donor. The enzyme differs from other 2'-deoxyribonucleoside kinases [EC 2.7.1.76 (2'-deoxyadenosine kinase) and EC 2.7.1.113 (deoxyguanosine kinase)] in its broad specificity for all four common deoxynucleosides. The quasi-irreversible transfer of phosphate from the high energy donor to the nucleoside enables high NMP yields.
EC Number
2.7.1.145
CAS Number
52227-81-3
Catalyzed reaction(s)
ATP + 2'-deoxyribonucleoside = ADP + 2'-deoxyribonucleoside 5'-phosphate
Synonyms
Dm-dNK, dNK, multispecific deoxynucleoside kinase, multisubstrate deoxyribonucleoside kinase, multifunctional deoxynucleoside kinase, ATP:deoxyribonucleoside 5'-phosphotransferase
Origin
Drosophila melanogaster
Molecular weight
30.5 kDa
Other characteristics
Mesophilic, broad substrate spectrum
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM deoxycytidine
6 mM ATP
0.04 g/l NK12 enzyme
37°C, 30 min
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Deoxycytidine kinase NK13
Art.-No.: E-KI-0113
Human DCK, mesophilic
180,00 €
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The deoxycytidine kinase NK13 originates from the human DCK gene. It predominantly converts deoxycytidine to dCMP, but also accepts deoxyguanosine and deoxyadenosine as substrates. The enzyme has broad substrate specificity and does not display selectivity based on the chirality of the substrate. It is an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. All natural nucleoside triphosphates (except dCTP) can act as donors. The quasi-irreversible transfer of phosphate from the high energy phosphate donor to the nucleoside enables high NMP yields.
EC Number
2.7.1.74
CAS Number
9039-45-6
Catalyzed reaction(s)
NTP + deoxycytidine = NDP + dCMP
Synonyms
hDCK, dCK, 2'-deoxycytidine kinase, Ara-C kinase, arabinofuranosylcytosine kinase, NTP:deoxycytidine 5'-phosphotransferase
Origin
Homo sapiens
Molecular weight
31.9 kDa
Other characteristics
Mesophilic, broad substrate spectrum
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM deoxycytidine
6 mM ATP
0.04 g/l NK13 enzyme
37°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Adenosine kinase NK14
Art.-No.: E-KI-0114
Human ADK, mesophilic
180,00 €
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The adenosine kinase NK14 originates from the human ADK gene. It catalyzes the phosphorylation of the purine nucleoside adenosine to AMP and also accepts deoxyadenosine and other related nucleoside analogs as substrates. The quasi-irreversible transfer of phosphate from the high energy phosphate donor to the nucleoside enables high NMP yields.
EC Number
2.7.1.20
CAS Number
9027-72-9
Catalyzed reaction(s)
ATP + adenosine = ADP + AMP
Synonyms
hADK, AK, ATP:adenosine 5'-phosphotransferase
Origin
Homo sapiens
Molecular weight
40.1 kDa
Other characteristics
Mesophilic, specific for adenosine-related substrates
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM adenosine
6 mM ATP
0.04 g/l NK14 enzyme
37°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Uridine/cytidine kinase NK15
Art.-No.: E-KI-0115
Human UCK2 (isoform 1), mesophilic
180,00 €
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The uridine/cytidine kinase NK15 originates from the human UCK2 gene (isoform 1). The enzyme phosphorylates both uridine and cytidine (and their analogs) to their respective monophosphate forms. It does not phosphorylate deoxyribonucleosides or purine ribonucleosides. The quasi-irreversible transfer of phosphate from the high energy phosphate donor to the nucleoside enables high NMP yields.
EC Number
2.7.1.48
CAS Number
9026-39-5
Catalyzed reaction(s)
(1) ATP + uridine = ADP + UMP
(2) ATP + cytidine = ADP + CMP
Synonyms
hUCK2, UCK, URK1, pyrimidine ribonucleoside kinase, uridine-cytidine kinase, uridine kinase, uridine phosphokinase, ATP:uridine 5'-phosphotransferase, ATP:uridine/cytidine 5'-phosphotransferase
Origin
Homo sapiens
Molecular weight
30.7 kDa
Other characteristics
Mesophilic, broad acceptance of pyrimidine ribonucleotides
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM uridine
6 mM ATP
0.04 g/l NK15 enzyme
37°C, 1 h
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Thymidine kinase NK16
Art.-No.: E-KI-0116
Viral origin, mesophilic
180,00 €
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The thymidine kinase NK16 is a mesophilic enzyme of viral origin. The enzyme phosphorylates thymidine, uridine, deoxyuridine, deoxycytidine and deoxyguanosine as well as a number of modified pyrimidine and purine derivatives. ATP or dGTP can act as phosphate donor. The quasi-irreversible transfer of phosphate from the high energy phosphate donor to the nucleoside enables high NMP yields.
EC Number
2.7.1.21
CAS Number
9002-06-6
Catalyzed reaction(s)
ATP + thymidine = ADP + dTMP
Synonyms
TK, 2'-deoxythymidine kinase, ATP:thymidine 5'-phosphotransferase
Origin
Viral
Molecular weight
42.2 kDa
Other characteristics
Mesophilic
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM thymidine
6 mM ATP
0.04 g/l NK16 enzyme
37°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
NMP kinases
Adenylate kinase NMPK01
Art.-No.: E-KI-0201
Bacterial origin, thermostable up to 80°C
160,00 €
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The adenylate kinase NMPK01 is of bacterial origin and thermostable up to 80°C. Adenylate kinases catalyze the phosphorylation of AMP or dAMP to ADP and dADP, respectively.
EC Number
2.7.4.3
CAS Number
9013-02-9
Catalyzed reaction(s)
ATP + AMP = 2 ADP
Synonyms
ADK, AMPK, adenylic kinase, adenylokinase, 5'-AMP-kinase, myokinase, ATP:AMP phosphotransferase
Origin
Bacterial
Molecular weight
26.4 kDa
Other characteristics
Thermostable up to 80°C
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 50°C)
10 mM MgCl2
5 mM AMP
6 mM dATP
0.04 g/l NMPK01 enzyme
50°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
(d)CMP kinase NMPK02
Art.-No.: E-KI-0202
Bacterial origin, thermostable up to 80°C
160,00 €
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The prokaryotic (d)CMP kinase NMPK02 specifically phosphorylates CMP (or dCMP), using ATP as the preferred phosphoryl donor. Unlike EC 2.7.4.14, a eukaryotic enzyme that phosphorylates UMP and CMP with similar efficiency, the prokaryotic enzyme phosphorylates UMP (and AMP) with much lower rates. The enzyme accepts dCMP nearly as well as it does CMP. NMPK02 is thermostable up to 80°C.
EC Number
2.7.4.25
CAS Number
N/A
Catalyzed reaction(s)
ATP + (d)CMP = ADP + (d)CDP
Synonyms
CMPK, dCMPK, prokaryotic cytidylate kinase, deoxycytidylate kinase, deoxycytidine monophosphokinase, ATP:(d)CMP phosphotransferase
Origin
Bacterial
Molecular weight
26.0 kDa
Other characteristics
Thermostable up to 80°C
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 50°C)
10 mM MgCl2
5 mM CMP
6 mM ATP
0.04 g/l NMPK02 enzyme
50°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Guanylate kinase NMPK03
Art.-No.: E-KI-0203
Bacterial origin, thermostable up to 80°C
160,00 €
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The prokaryotic guanylate kinase NMPK03 catalyzes the phosphorylation of GMP, dGMP, AMP and, to a small extent, dAMP. ATP and dATP can act as donor. NMPK03 is thermostable up to 80°C.
EC Number
2.7.4.8
CAS Number
9026-59-9
Catalyzed reaction(s)
ATP + GMP = ADP + GDP
Synonyms
GMPK, deoxyguanylate kinase, guanosine monophosphate kinase, 5'-GMP kinase, ATP:GMP phosphotransferase, ATP:(d)GMP phosphotransferase
Origin
Bacterial
Molecular weight
25.4 kDa
Other characteristics
Thermostable up to 80°C
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 50°C)
10 mM MgCl2
5 mM GMP
6 mM ATP
0.04 g/l NMPK03 enzyme
50°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
TMP kinase NMPK04
Art.-No.: E-KI-0204
Bacterial origin, thermostable up to 80°C
160,00 €
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The prokaryotic TMP kinase NMPK04 catalyzes the phosphorylation of (d)TMP, dGMP, AMP and, to a smaller extent, GMP. The enzyme is thermostable up to 80°C.
EC Number
2.7.4.9
CAS Number
9014-43-1
Catalyzed reaction(s)
ATP + dTMP = ADP + dTDP
Synonyms
TMPK, thymidine monophosphate kinase, thymidylate kinase, deoxythymidine 5'-monophosphate kinase, dTMPK, dTMP kinase, thymidylic acid kinase, thymidylic kinase, ATP:dTMP phosphotransferase
Origin
Bacterial
Molecular weight
24.2 kDa
Other characteristics
Thermostable up to 80°C
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 50°C)
10 mM MgCl2
5 mM dTMP
6 mM ATP
0.04 g/l NMPK04 enzyme
50°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
UMP kinase NMPK05
Art.-No.: E-KI-0205
Bacterial origin, thermostable up to 80°C
160,00 €
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The prokaryotic UMP kinase NMPK05 is specific for UMP as substrate and is used by prokaryotes in the de novo synthesis of pyrimidines, in contrast to eukaryotes, which use the dual-specificity enzyme UMP/CMP kinase (EC 2.7.4.14) for the same purpose. Secondary substrates are GMP and - to a much lesser extent - CMP. This enzyme is the subject of feedback regulation, being inhibited by UTP and activated by GTP. NMPK05 is thermostable up to 80°C.
EC Number
2.7.4.22
CAS Number
9036-23-1
Catalyzed reaction(s)
ATP + UMP = ADP + UDP
Synonyms
UMPK, uridylate kinase, uridine monophosphate kinase, ATP:UMP phosphotransferase
Origin
Bacterial
Molecular weight
26.6 kDa
Other characteristics
Thermostable up to 80°C
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 50°C)
10 mM MgCl2
5 mM UMP
6 mM ATP
0.04 g/l NMPK05 enzyme
50°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
TMP kinase NMPK06
Art.-No.: E-KI-0206
Eukaryotic origin, mesophilic
180,00 €
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The TMP kinase NMPK06 is a mesophilic enzyme of eukaryotic origin. It catalyzes the phosphorylation of TMP and dGMP as well as (to a smaller extent) dUMP, dIMP, GMP and a number of modified pyrimidine and purine derivatives. ATP and dATP can act as phosphate donors.
EC Number
2.7.4.9
CAS Number
9014-43-1
Catalyzed reaction(s)
ATP + dTMP = ADP + dTDP
Synonyms
TMPK, thymidine monophosphate kinase, thymidylate kinase, deoxythymidine 5'-monophosphate kinase, dTMPK, dTMP kinase, thymidylic acid kinase, thymidylic kinase, ATP:dTMP phosphotransferase
Origin
Eukaryotic
Molecular weight
26.0 kDa
Other characteristics
Mesophilic
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM dTMP
6 mM ATP
0.04 g/l NMPK06 enzyme
37°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
UMP kinase NMPK07
Art.-No.: E-KI-0207
Eukaryotic origin, mesophilic
180,00 €
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The UMP kinase NMPK07 is a mesophilic enzyme of eukaryotic origin. It catalyzes the phosphorylation of UMP and dUMP as well as (to a smaller extent) GMP, dGMP and TMP. ATP and dATP can act as phosphate donors.
EC Number
2.7.4.22
CAS Number
9036-23-1
Catalyzed reaction(s)
ATP + UMP = ADP + UDP
Synonyms
UMPK, uridylate kinase, uridine monophosphate kinase, ATP:UMP phosphotransferase
Origin
Eukaryotic
Molecular weight
24.2 kDa
Other characteristics
Mesophilic
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM UMP
6 mM ATP
0.04 g/l NMPK07 enzyme
37°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
dNMP kinase NMPK09
Art.-No.: E-KI-0209
Viral origin, mesophilic
160,00 €
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The dNMP kinase NMPK09 is a mesophilic enzyme of viral origin. It catalyzes the phosphorylation of dTMP, dGMP and some nucleotide analogs. ATP and dATP can act as phosphate donors.
EC Number
2.7.4.13
CAS Number
37278-20-9
Catalyzed reaction(s)
(1) ATP + dTMP = ADP + dTDP
(2) ATP + dGMP = ADP + dGDP
Synonyms
dNMPK, deoxynucleoside monophosphate kinase, ATP:deoxynucleoside-phosphate phosphotransferase
Origin
Viral
Molecular weight
28.6 kDa
Other characteristics
Mesophilic
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM dTMP
6 mM ATP
0.04 g/l NMPK09 enzyme
37°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Adenylate kinase NMPK10
Art.-No.: E-KI-0210
Bacterial origin, thermostable up to 60°C
220,00 €
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The adenylate kinase NMPK10 is of bacterial origin and thermostable up to 60°C. Adenylate kinases catalyze the phosphorylation of AMP or dAMP to ADP and dADP, respectively.
EC Number
2.7.4.3
CAS Number
9013-02-9
Catalyzed reaction(s)
ATP + AMP = 2 ADP
Synonyms
ADK, AMPK, adenylic kinase, adenylokinase, 5'-AMP-kinase, myokinase, ATP:AMP phosphotransferase
Origin
Bacterial
Molecular weight
25.4 kDa
Other characteristics
Thermostable up to 60°C
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM AMP
6 mM dATP
0.04 g/l NMPK10 enzyme
37°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Guanylate kinase NMPK21
Art.-No.: E-KI-0221
Eukaryotic origin, mesophilic
180,00 €
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The guanylate kinase NMPK21 is a mesophilic enzyme of eukaryotic origin. It catalyzes the phosphorylation of GMP or dGMP to GDP and dGDP, respectively. ATP and dATP can act as phosphate donor.
EC Number
2.7.4.8
CAS Number
9026-59-9
Catalyzed reaction(s)
ATP + GMP = ADP + GDP
Synonyms
GMPK, dGMPK, deoxyguanylate kinase, GMP kinase, guanosine monophosphate kinase, 5'-GMP kinase, ATP:GMP phosphotransferase
Origin
Eukaryotic
Molecular weight
22.0 kDa
Other characteristics
Mesophilic
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM GMP
6 mM ATP
0.04 g/l NMPK21 enzyme
37°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
UMP/CMP kinase NMPK22
Art.-No.: E-KI-0221
Human CMPK1, mesophilic
180,00 €
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The UMP/CMP kinase NMPK22 corresponds to the human gene CMPK1. This eukaryotic enzyme is a bifunctional enzyme that catalyzes the phosphorylation of both CMP and UMP with similar efficiency. dCMP can also act as acceptor. The enzyme plays an important role in de novo pyrimidine nucleotide biosynthesis.
EC Number
2.7.4.14
CAS Number
37278-21-0
Catalyzed reaction(s)
(1) ATP + (d)CMP = ADP + (d)CDP
(2) ATP + UMP = ADP + UDP
Synonyms
hCMPK1, U/CMPK, UCK, cytidylate kinase, cytidine monophosphate kinase, CMP kinase, CTP:CMP phosphotransferase, deoxycytidylate kinase, deoxycytidine monophosphate kinase, dCMP kinase, uridylate kinase, uridine monophosphate kinase, pyrimidine nucleoside monophosphate kinase, uridine monophosphate-cytidine monophosphate phosphotransferase, ATP:UMP-CMP phosphotransferase
Origin
Homo sapiens
Molecular weight
23.6 kDa
Other characteristics
Mesophilic
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM CMP
6 mM ATP
0.04 g/l NMPK22 enzyme
37°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
NDP kinases
Nucleoside-diphosphate kinase NDPK32
Art.-No.: E-KI-0332
Bacterial origin, thermostable up to 50°C
160,00 €
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The nucleoside-diphosphate kinase NDPK32 is of bacterial origin and thermostable up to 50°C. It catalyzes the phosphorylation of nucleoside diphosphates to nucleoside triphosphates.
EC Number
2.7.4.6
CAS Number
9026-51-1
Catalyzed reaction(s)
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
Synonyms
NDPK, nucleoside 5'-diphosphate kinase, nucleoside diphosphokinase, nucleotide phosphate kinase, NDP kinase, ATP:nucleoside-diphosphate phosphotransferase
Origin
Bacterial
Molecular weight
16.6 kDa
Other characteristics
Thermostable up to 50°C, broad substrate spectrum
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM GDP
6 mM ATP
0.04 g/l NDPK32 enzyme
50°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Nucleoside-diphosphate kinase NDPK33
Art.-No.: E-KI-0333
Human NME2, mesophilic
180,00 €
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The nucleoside-diphosphate kinase NDPK32 corresponds to the human gene NME2. It catalyzes the phosphorylation of nucleoside diphosphates to nucleoside triphosphates.
EC Number
2.7.4.6
CAS Number
9026-51-1
Catalyzed reaction(s)
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
Synonyms
hNME2, NDPK, nucleoside 5'-diphosphate kinase, nucleoside diphosphokinase, nucleotide phosphate kinase, NDP kinase, ATP:nucleoside-diphosphate phosphotransferase
Origin
Homo sapiens
Molecular weight
18.7 kDa
Other characteristics
Mesophilic, broad substrate spectrum
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM GDP
6 mM ATP
0.04 g/l NDPK33 enzyme
37°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Polyphosphate kinases
Polyphosphate kinase PPK03
Art.-No.: E-KI-0703
Bacterial PPK2 class I, mesophilic
580,00 €
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The polyphosphate kinase PPK03 is a bacterial PPK2 (class I) enzyme. Polyphosphate kinases of the PPK2 (class I) family preferentially catalyze polyphosphate-driven phosphorylation (utilization of polyphosphate) of nucleoside diphosphates and can thus subtitute nucleoside-diphosphate kinases or be used as ATP regeneration system in kinase reactions.
EC Number
2.7.4.1
CAS Number
9026-44-2
Catalyzed reaction(s)
ADP + (phosphate)n = ATP + (phosphate)n-1
Synonyms
PPK, PPK2 class I, polyphosphoric acid kinase, ATP:polyphosphate phosphotransferase
Origin
Bacterial
Molecular weight
32.4 kDa
Other characteristics
Mesophilic, can be used for ATP regeneration
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM ADP
6 mM polyphosphate
0.04 g/l PPK03 enzyme
37°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Polyphosphate kinase PPK04
Art.-No.: E-KI-0704
Bacterial PPK2 class III, thermostable up to 50°C
580,00 €
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The polyphosphate kinase PPK04 is a bacterial PPK2 (class III) enzyme and thermostable up to 50°C. Polyphosphate kinases of the PPK2 (class III) family preferentially catalyze polyphosphate-driven phosphorylation (utilization of polyphosphate) of both nucleoside monophosphates and nucleoside diphosphates and can thus subtitute NMPKs and NDPKs or be used as ATP regeneration system in kinase reactions. Hyperphosphorylation of nucleotides beyond three phosphate groups may occur.
EC Number
2.7.4.1
CAS Number
9026-44-2
Catalyzed reaction(s)
ADP + (phosphate)n = ATP + (phosphate)n-1
Synonyms
PPK, PPK2 class III, polyphosphoric acid kinase, ATP:polyphosphate phosphotransferase
Origin
Bacterial
Molecular weight
31.7 kDa
Other characteristics
Thermostable up to 50°C, can be used for ATP regeneration
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 50°C)
10 mM MgCl2
5 mM GMP
6 mM polyphosphate
0.04 g/l PPK04 enzyme
50°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Polyphosphate kinase PPK05
Art.-No.: E-KI-0705
Bacterial PPK2 (class III), thermostable up to 50°C
160,00 €
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The polyphosphate kinase PPK05 is a bacterial PPK2 (class III) enzyme and thermostable up to 50°C. Polyphosphate kinases of the PPK2 (class III) family preferentially catalyze polyphosphate-driven phosphorylation (utilization of polyphosphate) of both nucleoside monophosphates and nucleoside diphosphates and can thus substitute NMPKs and NDPKs or be used as ATP regeneration system in kinase reactions. Hyperphosphorylation of nucleotides beyond three 5'-phosphate groups may occur.
EC Number
2.7.4.1
CAS Number
9026-44-2
Catalyzed reaction(s)
(1) ADP + (phosphate)n = ATP + (phosphate)n-1
(2) AMP + (phosphate)n = ADP + (phosphate)n-1
Synonyms
PPK2 class III, polyphosphoric acid kinase, polyP kinase, ATP:polyphosphate phosphotransferase
Origin
Bacterial
Molecular weight
32.8 kDa
Other characteristics
Thermostable up to 50°C, can be used for ATP regeneration
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM AMP
6 mM polyphosphate
0.04 g/l PPK05 enzyme
37°C, 2 h
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Polyphosphate kinase PPK07
Art.-No.: E-KI-0707
Bacterial PPK2 (class III), mesophilic
180,00 €
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The polyphosphate kinase PPK07 is a mesophilic bacterial PPK2 (class III) enzyme. Polyphosphate kinases of the PPK2 (class III) family preferentially catalyze polyphosphate-driven phosphorylation (utilization of polyphosphate) of both nucleoside monophosphates and nucleoside diphosphates and can thus substitute NMPKs and NDPKs or be used as ATP regeneration system in kinase reactions. Hyperphosphorylation of nucleotides beyond three 5'-phosphate groups may occur.
EC Number
2.7.4.1
CAS Number
9026-44-2
Catalyzed reaction(s)
(1) ADP + (phosphate)n = ATP + (phosphate)n-1
(2) AMP + (phosphate)n = ADP + (phosphate)n-1
Synonyms
PPK2 class III, polyphosphoric acid kinase, polyP kinase, ATP:polyphosphate phosphotransferase
Origin
Bacterial
Molecular weight
33.4 kDa
Other characteristics
Mesophilic, can be used for ATP regeneration
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM AMP
6 mM polyphosphate
0.04 g/l PPK07 enzyme
37°C, 2 h
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Polyphosphate kinase PPK08
Art.-No.: E-KI-0708
Bacterial PPK2 (class III), mesophilic
180,00 €
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The polyphosphate kinase PPK08 is a mesophilic bacterial PPK2 (class III) enzyme. Polyphosphate kinases of the PPK2 (class III) family preferentially catalyze polyphosphate-driven phosphorylation (utilization of polyphosphate) of both nucleoside monophosphates and nucleoside diphosphates and can thus substitute NMPKs and NDPKs or be used as ATP regeneration system in kinase reactions. Hyperphosphorylation of nucleotides beyond three 5'-phosphate groups may occur.
EC Number
2.7.4.1
CAS Number
9026-44-2
Catalyzed reaction(s)
(1) ADP + (phosphate)n = ATP + (phosphate)n-1
(2) AMP + (phosphate)n = ADP + (phosphate)n-1
Synonyms
PPK2 class III, polyphosphoric acid kinase, polyP kinase, ATP:polyphosphate phosphotransferase
Origin
Bacterial
Molecular weight
34.3 kDa
Other characteristics
Mesophilic, can be used for ATP regeneration
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM AMP
6 mM polyphosphate
0.04 g/l PPK08 enzyme
37°C, 2 h
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Other kinases
Acetate kinase AcK01
Art.-No.: E-KI-0401
Bacterial origin, thermostable up to 80°C
220,00 €
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The acetate kinase AcK01 is a thermostable enzyme of bacterial origin. It phosphorylates all natural (d)NDPs to the respective (d)NTPs using acetyl phosphate as phosphate donor and can be used as a substitute for nucleoside-diphosphate kinases and as an ATP regeneration system in kinase reactions.
EC Number
2.7.2.1
CAS Number
9027-42-3
Catalyzed reaction(s)
ADP + acetyl phosphate = ATP + acetate
Synonyms
AcK, acetokinase, AckA, AK, acetic kinase, ATP:acetate phosphotransferase
Origin
Bacterial
Molecular weight
46.1 kDa
Other characteristics
Thermostable up to 80°C, can be used for ATP regeneration
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM ADP
6 mM acetyl phosphate
0.04 g/l AcK01 enzyme
37°C, 2 h
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Creatine kinase (M-type) CK01
Art.-No.: E-KI-0501
Human CKM, mesophilic
220,00 €
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The creatine kinase (M-type) CK01 corresponds to the human CKM gene. The enzyme reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). CK01 can be used as ATP regeneration system in kinase reactions.
EC Number
2.7.3.2
CAS Number
9001-15-4
Catalyzed reaction(s)
ATP + creatine = ADP + H+ + N-phosphocreatine
Synonyms
hCKM, CK, creatine kinase muscle, M-CK, creatine kinase M chain, creatine phosphokinase M-type, CPK-M, ATP:creatine phosphotransferase
Origin
Homo sapiens
Molecular weight
69.3 kDa (including GST-Tag)
Other characteristics
Mesophilic, can be used for ATP regeneration
Production
Recombinant from E. coli, purified by GST-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM ADP
6 mM creatine phosphate
0.04 g/l CK01 enzyme
37°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Pyruvate kinase PK01
Art.-No.: E-KI-0601
Archaeal origin, thermostable up to (at least) 80°C
220,00 €
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The pyruvate kinase PK01 is of archaeal origin and thermostable up to (at least) 80°C. Pyruvate kinases phosphorylate (d)NDPs in a quasi irreversible manner using phosphoenolpyruvate as phosphate donor. They can be used as (d)NTP regeneration system in kinase reactions.
EC Number
2.7.1.40
CAS Number
9001-59-6
Catalyzed reaction(s)
ATP + pyruvate = ADP + phosphoenolpyruvate
Synonyms
PK, PEPK phosphoenolpyruvate kinase, phosphoenol transphosphorylase, fluorokinase, pyruvic kinase, pyruvate phosphotransferase, ATP:pyruvate 2-O-phosphotransferase
Origin
Archaeal
Molecular weight
51.9 kDa
Other characteristics
Thermostable up to (at least) 80°C, can be used for ATP regeneration
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM ADP
6 mM phosphoenolpyruvate
0.04 g/l PK01 enzyme
37°C, 2 h
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Pyruvate kinase PK02
Art.-No.: E-KI-0602
Bacterial origin, thermostable up to 80°C
180,00 €
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The pyruvate kinase PK02 is of bacterial origin and thermostable up to 80°C. Pyruvate kinases phosphorylate pyruvate to phosphoenolpyruvate using ATP, UTP, GTP, CTP, ITP or dATP as phosphate donor. By utilizing the reverse reaction PK02 can be used as ATP regeneration system in kinase reactions. Substrate preference: ADP, dADP, GDP, dGDP >> UDP > TDP, CDP, dCDP.
EC Number
2.7.1.40
CAS Number
9001-59-6
Catalyzed reaction(s)
ATP + pyruvate = ADP + phosphoenolpyruvate
Synonyms
PK, phosphoenolpyruvate kinase, phosphoenol transphosphorylase, fluorokinase, pyruvic kinase, pyruvate phosphotransferase, ATP:pyruvate 2-O-phosphotransferase
Origin
Bacterial
Molecular weight
51.9 kDa
Other characteristics
Thermostable up to 80°C, can be used for ATP regeneration
Production
Recombinant from E. coli, purified by heat treatment and His-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 50°C)
10 mM MgCl2
5 mM ADP
6 mM phosphoenolpyruvate
0.04 g/l PK02 enzyme
50°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Glucose/glucosamine kinase (ADP-specific) GK01
Art.-No.: E-KI-0801
Archaeal origin, thermostable up to 80°C
220,00 €
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The ADP-specific glucose/glucosamine kinase GK01 is of archaeal origin and thermostable up to (at least) 80°C. This enzyme catalyzes the phosphorylation of glucose or glucosamine to glucose 6-phosphate and glucosamine 6-phosphate, respectively. It is highly specific for ADP as phosphate donor. No activity can be detected when ADP is replaced by ATP, GDP, phosphoenolpyruvate, diphosphate or polyphosphate.
EC Number
2.7.1.147
CAS Number
173585-07-4
Catalyzed reaction(s)
(1) ADP + D-glucose = AMP + D-glucose 6-phosphate
(2) ADP + D-glucosamine = AMP + D-glucosamine 6-phosphate
Synonyms
GK, ADP-specific glucokinase, ADP-dependent glucokinase, ADP:D-glucose/D-glucosamine 6-phosphotransferase
Origin
Archaeal
Molecular weight
52.5 kDa
Other characteristics
Thermostable up to (at least) 80°C
Production
Recombinant from E. coli, purified by heat treatment and His-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Ribokinase RK01
Art.-No.: E-KI-0901
Escherichia coli rbsK, mesophilic
290,00 €
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The ribokinase RK01 corresponds to the Escherichia coli rbsK gene. Ribokinases catalyze the phosphorylation of ribose (and its derivatives) at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for synthesis of nucleotides, histidine and tryptophan, or as a component of the pentose phosphate pathway.
EC Number
2.7.1.15
CAS Number
9026-84-0
Catalyzed reaction(s)
ATP + D-ribose = ADP + D-ribose 5-phosphate
Synonyms
RbsK, RK, deoxyribokinase, D-ribokinase, ATP:D-ribose 5-phosphotransferase
Origin
Escherichia coli
Molecular weight
33.7 kDa
Other characteristics
Mesophilic
Production
Recombinant from E. coli, purified by His-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Ribokinase RK02
Art.-No.: E-KI-0902
Bacterial origin, thermostable up to 60°C
290,00 €
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The ribokinase RK02 is of bacterial origin and thermostable up to 60°C. Ribokinases catalyze the phosphorylation of ribose (and its derivatives) at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for synthesis of nucleotides, histidine and tryptophan, or as a component of the pentose phosphate pathway.
EC Number
2.7.1.15
CAS Number
9026-84-0
Catalyzed reaction(s)
ATP + D-ribose = ADP + D-ribose 5-phosphate
Synonyms
RK, deoxyribokinase, D-ribokinase, ATP:D-ribose 5-phosphotransferase
Origin
Bacterial
Molecular weight
33.1 kDa
Other characteristics
Thermostable up to 60°C
Production
Recombinant from E. coli, purified by heat treatment and His-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Ribokinase RK03
Art.-No.: E-KI-0903
Bacterial origin, thermostable up to 80°C
290,00 €
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The ribokinase RK03 is of bacterial origin and thermostable up to 80°C. Ribokinases catalyze the phosphorylation of ribose (and its derivatives) at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for synthesis of nucleotides, histidine and tryptophan, or as a component of the pentose phosphate pathway.
EC Number
2.7.1.15
CAS Number
9026-84-0
Catalyzed reaction(s)
ATP + D-ribose = ADP + D-ribose 5-phosphate
Synonyms
RK, deoxyribokinase, D-ribokinase, ATP:D-ribose 5-phosphotransferase
Origin
Bacterial
Molecular weight
33.9 kDa
Other characteristics
Thermostable up to 80°C
Production
Recombinant from E. coli, purified by heat treatment and His-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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