Enzymes
Nucleotidyltransfer
Poly(U) polymerase PUP01
E-PP-0001
Eukaryotic origin, mesophilic
140,00 €
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- Available
- Ships within 15-30 working days
The poly(U) polymerase PUP01 is a mesophilic enzyme of eukaryotic origin. The enzyme catalyzes the terminal uridylation of mRNAs with short poly(U) tails, hence facilitating global mRNA decay. PUP requires an oligoribonucleotide or polyribonucleotide with a free terminal 3'-OH as a primer. Also possesses a weak poly(A) polymerase (PAP) activity.
EC Number
2.7.7.52
CAS Number
78519-53-6
Catalyzed reaction(s)
(1) UTP + RNAn = diphosphate + RNAn+1
(2) ATP + RNAn = diphosphate + RNAn+1
Synonyms
PUP, Cid1, RNA uridylyltransferase, terminal uridylyltransferase, TUT, TUTase, non-canonical poly(A) polymerase, UTP:RNA uridylyltransferase
Origin
Eukaryotic
Molecular weight
47.7 kDa
Other characteristics
Mesophilic, additional weak PAP activity
Production
Recombinant from E. coli, purified by His-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Terminal deoxynucleoside transferase TdT01
E-TT-0001
Eukaryotic origin, mesophilic
140,00 €
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- Available
- Ships within 15-30 working days
The terminal deoxynucleoside transferase TdT01 is a mesophilic enzyme of eukaryotic origin. This enzyme is a template-independent DNA polymerase which catalyzes the random addition of a deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator one nucleotide at a time. It cannot initiate a chain de novo. Also accepts ribonucleotides.
EC Number
2.7.7.31
CAS Number
9027-67-2
Catalyzed reaction(s)
2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1
Synonyms
TdT, Dntt, DNA nucleotidylexotransferase, terminal deoxyribonucleotidyltransferase, terminal addition enzyme, deoxynucleotidyl terminal transferase, deoxyribonucleic acid nucleotidyltransferase, deoxyribonucleic nucleotidyltransferase, terminal deoxynucleotide transferase, 2'-deoxyribonucleoside-5'-triphosphate:DNA deoxynucleotidylexotransferase
Origin
Eukaryotic
Molecular weight
59.9 kDa
Other characteristics
Mesophilic
Production
Recombinant from E. coli, purified by His-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
