NDP Kinase NDPK-32,
Regeneration Kinase RK,
10x Phosphorylation & Regeneration Buffer,
Regeneration Phosphate Donor
The NDP Kinase Kit + Phosphate Regeneration System was designed to enzymatically phosphorylate a wide range of purine and pyrimidine nucleoside diphosphates using enzyme NDPK-32. To some extent nucleoside monophosphates are also accepted as substrates. The use of the phosphate donor regeneration system offers several advantages: a) Simplified analytics and purification. Regeneration of the phosphate donor will - in case of 100 % conversion - lead to a two-compound reaction solution only containing the synthesized phosphorylated substrate and ATP, whereas without regeneration also ADP and AMP will be present possibly interfering with HPLC-DAD analysis and/or purification. b) Free choice of substrate-phosphate donor ratio. Some enzymatic phosphorylation reactions require a low phosphate donor concentration. Ratios of substrate to phosphate donor lower than 1:1 will lead to a loss of yield if the phosphate donor is not regenerated during the reaction. Biochemical action of nucleoside diphosphate kinases: Nucleoside diphosphate kinases catalyze the following reaction: NDP + ATP ↔ NTP + ADP Unit definition: One unit of nucleoside kinase will cause the conversion of 1.0 μmol each of NDP and ATP to NTP and ADP per min at pH 7.6 at optimum 37°C. One unit of regeneration kinase will cause the conversion of 1.0 μmol each of ADP and regeneration phosphate donor to regeneration donor product and ATP per min at pH 7.6 at 37°C. Product use limitations: This product is developed and sold exclusively for research purposes and in vitro use only. The product was not tested for use in diagnostics or drug development, nor is suitable for administration to humans or animals.