Nucleoside phosphorylases

Nucleoside phosphorylases (NPs) are transferases catalyzing the reversible cleavage of the C–N glycosidic bond by a phosphate group to yield the free nucleobase and ribose-1-phosphate. Based on their substrate spectrum they can be classified into purine nucleoside phosphorylases (PNPs, EC 2.4.2.1) and pyrimidine nucleoside phosphorylases (PyNPs, EC 2.4.2.2).

 

PNPs catalyze the reversible phosphorolytic cleavage of purine nucleosides. Natural substrates are both adenine and guanine. PNPs of bacterial origin use a wide variety of modified nucleosides as substrates.

 

PNP reaction

 

PyNPs catalyze the reversible phosphorolytic cleavage of pyrimidine nucleosides. Natural substrates are both uridine and thymidine. PyNPs of bacterial origin use a wide variety of modified nucleosides as substrates. Cytidine, however, is not converted by pyrimidine nucleoside phosphorylases.

 

PyNP reaction

 

By combining a PNP and a PyNP, the enzymatic synthesis of natural and modified nucleosides is possible in a one-pot, two enzyme transglycosylation reaction.

If you need detailed information on the temperature activity range or suitable enzyme combinations please contact us or check out our enzyme activity screening service.

Transglycosylation reaction

Purine nucleoside phosphorylases available at BioNukleo:


Cat. No. Properties Amount Price
N01 Prokaryotic purine nucleoside phosphorylase 1, recombinant
Optimal temperature: 55°C
Active towards: purines with 2'-amino-, 2'-deoxy- or 2'- and/or 3'-halogenated sugars (read more)
400 µg 120,00 €
N02 Prokaryotic purine nucleoside phosphorylase 2, recombinant
Optimal temperature: 60 - 70°C
Active towards: purines with 2'-amino-, 2'-arabino-, 2'-fluoroarabino- or 2'- and/or 3'-halogenated sugars (read more)
400 µg 120,00 €
N08 Archaeal purine nucleoside phosphorylase, recombinant
Optimal temperature: 80°C
Active towards: purines with 2'-amino-, 2'-fluoroarabino- or 2'- and/or 3'-halogenated sugars (read more)
400 µg 120,00 €
N11 E. coli purine nucleoside phosphorylase, recombinant
Optimal temperature: 37°C
Preferred natural substrate: inosine (read more)
400 µg 120,00 €

Pyrimidine nucleoside phosphorylases available at BioNukleo:


Cat. No. Properties Amount Price
Y01 Prokaryotic pyrimidine nucleoside phosphorylase 1, recombinant
Optimal temperature: 50°C
Active towards: pyrimidines with 2'-amino- or 2'-deoxysugars or with ethynyl-substituents
Active at wide temperature range (read more)
400 µg 120,00 €
Y02 Prokaryotic pyrimidine nucleoside phosphorylase 2, recombinant
Optimal temperature: 60°C
Active towards: pyrimidines with 2'-amino- or 2'-arabinosugars (read more)
400 µg 120,00 €
Y04 Prokaryotic pyrimidine nucleoside phosphorylase 4, recombinant
Optimal temperature: 80°C
Active towards: pyrimidines with 2'-amino-, 2'-arabino- or 2'-deoxysugars or with ethynyl-substituents (read more)
400 µg 120,00 €
Y07 Prokaryotic pyrimidine nucleoside phosphorylase 7, recombinant
Optimal temperature: 80°C
Active towards: pyrimidines with 2'-amino- or 2'-arabinosugars (read more)
400 µg 120,00 €
Y11 E. coli thymidine phosphorylase, recombinant
Optimal temperature: 37°C
Preferred natural substrate: thymidine (read more)
400 µg 120,00 €
Y12 E. coli uridine phosphorylase, recombinant
Optimal temperature: 37°C
Preferred natural substrate: uridine (read more)
400 µg 120,00 €

Enzyme kits available at BioNukleo:

 

Nucleoside phosphorylase Kit
Carefully assembled kit for the production of nucleosides. The kit includes a set of BioNukleo's thermostable nucleoside phosphorylases to screen for best activity on your compound of interest.   Modified nucleosides can be pro ... (Read More)
900,00 €