Nucleoside phosphorylases
Nucleoside phosphorylases (NPs) are transferases catalyzing the reversible cleavage of the C–N glycosidic bond by a phosphate group to yield the free nucleobase and ribose-1-phosphate. Based on their substrate spectrum they can be classified into purine nucleoside phosphorylases (PNPs, EC 2.4.2.1) and pyrimidine nucleoside phosphorylases (PyNPs, EC 2.4.2.2).
PNPs catalyze the reversible phosphorolytic cleavage of purine nucleosides. Natural substrates are both adenine and guanine. PNPs of bacterial origin use a wide variety of modified nucleosides as substrates.
PyNPs catalyze the reversible phosphorolytic cleavage of pyrimidine nucleosides. Natural substrates are both uridine and thymidine. PyNPs of bacterial origin use a wide variety of modified nucleosides as substrates. Cytidine, however, is not converted by pyrimidine nucleoside phosphorylases.
By combining a PNP and a PyNP, the enzymatic synthesis of natural and modified nucleosides is possible in a one-pot, two enzyme
transglycosylation reaction.
If you need detailed information on the temperature activity range or suitable enzyme combinations please contact us or check out our enzyme activity screening service.
Purine nucleoside phosphorylases available at BioNukleo:
| Cat. No. | Properties | Amount | Price |
|---|---|---|---|
| N01 |
Prokaryotic purine nucleoside phosphorylase 1, recombinant Optimal temperature: 55°C Active towards: purines with 2'-amino-, 2'-deoxy- or 2'- and/or 3'-halogenated sugars (read more) |
400 µg | 120,00 € |
| N02 |
Prokaryotic purine nucleoside phosphorylase 2, recombinant Optimal temperature: 60 - 70°C Active towards: purines with 2'-amino-, 2'-arabino-, 2'-fluoroarabino- or 2'- and/or 3'-halogenated sugars (read more) |
400 µg | 120,00 € |
| N08 |
Archaeal purine nucleoside phosphorylase, recombinant Optimal temperature: 80°C Active towards: purines with 2'-amino-, 2'-fluoroarabino- or 2'- and/or 3'-halogenated sugars (read more) |
400 µg | 120,00 € |
| N11 |
E. coli purine nucleoside phosphorylase, recombinant Optimal temperature: 37°C Preferred natural substrate: inosine (read more) |
400 µg | 120,00 € |
Pyrimidine nucleoside phosphorylases available at BioNukleo:
| Cat. No. | Properties | Amount | Price |
|---|---|---|---|
| Y01 |
Prokaryotic pyrimidine nucleoside phosphorylase 1, recombinant Optimal temperature: 50°C Active towards: pyrimidines with 2'-amino- or 2'-deoxysugars or with ethynyl-substituents Active at wide temperature range (read more) |
400 µg | 120,00 € |
| Y02 |
Prokaryotic pyrimidine nucleoside phosphorylase 2, recombinant Optimal temperature: 60°C Active towards: pyrimidines with 2'-amino- or 2'-arabinosugars (read more) |
400 µg | 120,00 € |
| Y04 |
Prokaryotic pyrimidine nucleoside phosphorylase 4, recombinant Optimal temperature: 80°C Active towards: pyrimidines with 2'-amino-, 2'-arabino- or 2'-deoxysugars or with ethynyl-substituents (read more) |
400 µg | 120,00 € |
| Y07 |
Prokaryotic pyrimidine nucleoside phosphorylase 7, recombinant Optimal temperature: 80°C Active towards: pyrimidines with 2'-amino- or 2'-arabinosugars (read more) |
400 µg | 120,00 € |
| Y11 |
E. coli thymidine phosphorylase, recombinant Optimal temperature: 37°C Preferred natural substrate: thymidine (read more) |
400 µg | 120,00 € |
| Y12 |
E. coli uridine phosphorylase, recombinant Optimal temperature: 37°C Preferred natural substrate: uridine (read more) |
400 µg | 120,00 € |