Nucleoside and nucleotide kinases

Nucleoside and nucleotide kinases are phospho-transferases catalyzing the addition of phosphate groups to the 5'-end of nucleosides or nucleotides. ATP or other NTPs can serve as phosphate donors.

 

Nucleoside kinases (NKs, EC 2.7.1.x) perform the irreversible transfer of a phosphate group from ATP (or other NTPs) to the 5'-end of nucleosides to form nucleoside monophosphates.

NK reaction

Nucleoside monophosphate kinases (NMPKs, EC 2.7.4.x) catalyze the reversible transfer of a phosphate group from ATP (or other NTPs) to the 5'-end of nucleoside monophosphates to form nucleoside diphosphates. Additionally, many NMPKs show little NDPK activity. 

Nucleoside diphosphate kinases (NDPKs, EC 2.7.4.6) catalyze the reversible transfer of a phosphate group from ATP (or other NTPs) to the 5'-end of nucleoside diphosphates to form nucleoside triphosphates.

Substrate spectra differ greatly between kinases. There are rather specific enzymes like thymidine kinase (TK, EC 2.7.1.21) from Escherichia coli as well as wide spectrum biocatalysts like deoxynucleoside kinase (dNK, EC 2.7.1.145) from Drosophila melanogaster which accepts all natural deoxynucleosides and many nucleoside analogs as substrates. Furthermore, the optimal reaction temperature ranges from moderate to high depending on the organism the enzyme originates from.

 

By combining a NK, a NMPK and a NDPK, the enzymatic synthesis of natural and modified nucleoside triphosphates (NTPs) is possible in a one-pot, three enzyme reaction. The incorporation of an ATP regeneration system might significantly enhance product yields and shorten reaction times.

If you need detailed information on the temperature activity range or suitable enzyme combinations please contact us or check out our enzyme activity screening service


Nucleoside kinases available at BioNukleo:


Cat. No. Properties Amount Price
NK13 Human deoxycytidine kinase, recombinant
Optimal temperature: 37°C
Active towards: pyrimidine nucleosides
Wide substrate spectrum (read more)
200 µg 160,00 €
NK14 Human adenosine kinase, recombinant
Optimal temperature: 37°C
Active towards: purine nucleosides (read more)
200 µg 160,00 €
NK15 Human uridine-cytidine kinase 2, recombinant
Optimal temperature: 37°C
Active towards: pyrimidine nucleosides (read more)
200 µg 160,00 €

Nucleoside monophosphate kinases available at BioNukleo:


Cat. No. Properties Amount Price
NMPK21 Eukaryotic guanylate kinase, recombinant
Optimal temperature: 37°C
Active towards: purine nucleoside monophosphates (read more)
200 µg 160,00 €
NMPK22 Human UMP-CMP kinase, recombinant
Optimal temperature: 37°C
Active towards: pyrimidine nucleoside monophosphates (read more)
200 µg 160,00 €
NMPK23 Human adenylate kinase 9, recombinant
Optimal temperature: 37°C
Active towards: purine nucleoside monophosphates (read more)
200 µg 160,00 €

Nucleoside diphosphate kinases available at BioNukleo:


Cat. No. Properties Amount Price
NDPK32 Prokaryotic nucleoside diphosphate kinase, recombinant
Optimal temperature: 50°C
Active towards: purine and pyrimidine nucleoside diphosphates (read more)
200 µg 160,00 €
NDPK33 Human nucleoside diphosphate kinase B, recombinant
Optimal temperature: 37°C
Active towards: purine and pyrimidine nucleoside diphosphates (read more)
200 µg 160,00 €