The thymidine phosphorylase Y11 is a mesophilic enzyme originating from Escherichia coli. Thymidine phosphorylases catalyze the reversible phosphorolytic breakdown of the N-glycosidic bond of thymidine with the formation of thymine and deoxyribose-1-phosphate.
DeoA, TP, TdRPase, Tpase, thymidine-orthophosphate deoxyribosyltransferase, deoxythymidine phosphorylase, dthdpase, pyrimidine phosphorylase, thymidine:phosphate deoxy-α-D-ribosyltransferase
thymidine + phosphate = thymine + 2-deoxy-α-D-ribose 1-phosphate
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Liquid, 50 % (v/v) glycerin
Example reaction conditions
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.