Pyrimidine nucleoside phosphorylase Y03
The pyrimidine nucleoside phosphorylase Y03 is of bacterial origin and thermostable up to 60°C. Pyrimidine nucleoside phosphorylases catalyze the reversible phosphorolytic breakdown of the N-glycosidic bond of the pyrimidine nucleosides uridine, thymidine and 2'-deoxyuridine with the formation of the corresponding pyrimidine base and pentose-1-phosphate. The enzyme does not distinguish between alpha-D-ribose 1-phosphate and 2-deoxy-alpha-D-ribose 1-phosphate in the synthetic direction.
E-NP-1003
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Product Information
EC Number
2.4.2.2
CAS Number
9055-35-0
Synonyms
PyNP, Py-NPase, PYNP, pynpase, pyrimidine ribonucleoside phosphorylase, pyrimidine-nucleoside:phosphate (2'-deoxy)-α-D-ribosyltransferase
Catalyzed reaction(s)
(1) uridine + phosphate = uracil + α-D-ribose 1-phosphate
(2) 2'-deoxyuridine + phosphate = uracil + 2-deoxy-α-D-ribose 1-phosphate
(3) thymidine + phosphate = thymine + 2-deoxy-α-D-ribose 1-phosphate
Properties
Origin
Bacterial
Molecular weight
46.8 kDa
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Thermostable up to 60°C
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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