The 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase H06 corresponds to the Escherichia coli mtnN gene. The enzyme catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively. It also cleaves 5'-deoxyadenosine, a toxic by-product of radical S-adenosylmethionine (SAM) enzymes, into 5-deoxyribose and adenine and accepts 5'-isobutylthioadenosine, 5'-n-butylthioadenosine, S-adenosyl-D-homocysteine, decarboxylated adenosylhomocysteine, deaminated adenosylhomocysteine and S-2-aza-adenosylhomocysteine as well as purine ribonucleosides in vitro.
Ec-MtnN, MTAN, SAHN, Ec-YadA, 5'-methyladenosine nucleosidase, S-methyl-5'-thioadenosine/S-adenosyl-L-homocysteine nucleosidase, S-adenosylhomocysteine hydrolase, S-adenosylhomocysteine nucleosidase, AdoHcy/MTA nucleosidase, S-adenosyl-L-homocysteine homocysteinylribohydrolase
(1) S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
(2) 5'-deoxyadenosine + H2O = 5-deoxy-D-ribose + adenine
(3) S-methyl-5'-thioadenosine + H2O = 5-(methylsulfanyl)-D-ribose + adenine
(4) purine nucleoside + H2O = D-ribose + purine base
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Liquid, 50 % (v/v) glycerin
Example reaction conditions
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.