H06 - BioNukleo

MTA/SAH nucleosidase H06

The 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase H06 corresponds to the Escherichia coli mtnN gene. The enzyme catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively. It also cleaves 5'-deoxyadenosine, a toxic by-product of radical S-adenosylmethionine (SAM) enzymes, into 5-deoxyribose and adenine and accepts 5'-isobutylthioadenosine, 5'-n-butylthioadenosine, S-adenosyl-D-homocysteine, decarboxylated adenosylhomocysteine, deaminated adenosylhomocysteine and S-2-aza-adenosylhomocysteine as well as purine ribonucleosides in vitro.

E-NH-0006

E. coli MtnN, mesophilic

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Product Information

EC Number

3.2.2.9

CAS Number

9055-10-1

Synonyms

Ec-MtnN, MTAN, SAHN, Ec-YadA, 5'-methyladenosine nucleosidase, S-methyl-5'-thioadenosine/S-adenosyl-L-homocysteine nucleosidase, S-adenosylhomocysteine hydrolase, S-adenosylhomocysteine nucleosidase, AdoHcy/MTA nucleosidase, S-adenosyl-L-homocysteine homocysteinylribohydrolase

Catalyzed reaction(s)

(1) S-adenosyl-L-homocysteine + H₂O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine

(2) 5'-deoxyadenosine + H₂O = 5-deoxy-D-ribose + adenine

(3) S-methyl-5'-thioadenosine + H₂O = 5-(methylsulfanyl)-D-ribose + adenine

(4) purine nucleoside + H₂O = D-ribose + purine base