The uridine hydrolase H04 is a mesophilic enzyme of eukaryotic origin. The enzyme catalyzes the hydrolysis of pyrimidine ribonucleosides to ribose and nucleobase with a clear preference for uridine.
UH, uridine nucleosidase, uridine ribohydrolase
uridine + H2O = D-ribose + uracil
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Liquid, 50 % (v/v) glycerin
Example reaction conditions
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.