The ribonucleoside hydrolase H02 corresponds to the Escherichia coli rihB gene. Ribonucleoside hydrolases catalyze the irreversible hydrolysis of nucleosides to ribose and nucleobase. H02 is stricktly specific for ribonucleosides and accepts the pyrimidine nucleosides cytidine and uridine with clear preference for cytidine over uridine. Purine ribonucleosides are also accepted, but conversion is much slower.
Ec-RihB, RNH, Ec-YeiK, ribosylpyrimidine nucleosidase, pyrimidine nucleoside hydrolase, N-ribohydrolase, N-ribosylpyrimidine nucleosidase, pyrimidine-nucleoside ribohydrolase
pyrimidine nucleoside + H2O = D-ribose + pyrimidine base
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Liquid, 50 % (v/v) glycerin
Example reaction conditions
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.