The polynucleotide kinase PNK01 is a modified version of the Escherichia virus T4 PseT enzyme. This enzyme catalyzes the phosphorylation of 5'-dephosphorylated DNA and also acts on 5'-dephosho-RNA 3'-mononucleotides. Three point mutations result in complete loss of 3' phosphatase activity.
PseT modified, PNK, ATP:5'-dephosphopolynucleotide 5'-phosphatase, PNK, polynucleotide 5'-hydroxyl kinase, 5'-hydroxyl polyribonucleotide kinase, 5'-hydroxyl RNA kinase, DNA 5'-hydroxyl kinase, DNA kinase, polynucleotide 5'-hydroxy-kinase, ATP:5'-dephosphopolynucleotide 5'-phosphotransferase
ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA
Escherichia virus T4
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Mesophilic, lacks 3'-phosphatase activity
Liquid, 50 % (v/v) glycerin
Example reaction conditions
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.