The ribokinase R01 corresponds to the Escherichia coli rbsK gene. Ribokinases catalyze the phosphorylation of ribose (and its derivatives) at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for synthesis of nucleotides, histidine and tryptophan, or as a component of the pentose phosphate pathway.
RbsK, RK, deoxyribokinase, D-ribokinase, ATP:D-ribose 5-phosphotransferase
ATP + D-ribose = ADP + D-ribose 5-phosphate
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Liquid, 50 % (v/v) glycerin
Example reaction conditions
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.