The polyphosphate kinase PPK01 corresponds to the Escherichia coli PPK1. Polyphosphate kinases of the PPK1 family catalyze the formation of polyphosphate from ATP. By utilizing the reverse reaction PPK01 can be used as ATP regeneration system in kinase reactions. The order of substrate specificity is ADP > GDP > UDP, CDP.
PPK1, PPK, polyphosphoric acid kinase, ATP:polyphosphate phosphotransferase
ATP + (phosphate)n = ADP + (phosphate)n+1
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Mesophilic, allows ATP regeneration
Liquid, 50 % (v/v) glycerin
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM ADP
6 mM polyphosphate
0.04 g/l PPK01 enzyme
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.