Polyphosphate kinase PPK01
The polyphosphate kinase PPK01 corresponds to the Escherichia coli PPK1. Polyphosphate kinases of the PPK1 family catalyze the formation of polyphosphate from ATP. By utilizing the reverse reaction PPK01 can be used as ATP regeneration system in kinase reactions. The order of substrate specificity is ADP > GDP > UDP, CDP.
E-KI-0701
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Product Information
EC Number
2.7.4.1
CAS Number
9026-44-2
Synonyms
PPK1, PPK, polyphosphoric acid kinase, ATP:polyphosphate phosphotransferase
Catalyzed reaction(s)
ATP + (phosphate)n = ADP + (phosphate)n+1
Properties
Origin
Escherichia coli
Molecular weight
82.1 kDa
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Mesophilic, allows ATP regeneration
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM ADP
6 mM polyphosphate
0.04 g/l PPK01 enzyme
37°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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