The pyruvate kinase PK02 is of bacterial origin and thermostable up to 80°C. Pyruvate kinases phosphorylate pyruvate to phosphoenolpyruvate using ATP, UTP, GTP, CTP, ITP or dATP as phosphate donor. By utilizing the reverse reaction PK02 can be used as ATP regeneration system in kinase reactions. Substrate preference: ADP, dADP, GDP, dGDP>>UDP>TDP, CDP, dCDP.
PK, phosphoenolpyruvate kinase, phosphoenol transphosphorylase, fluorokinase, pyruvic kinase, pyruvate phosphotransferase, ATP:pyruvate 2-O-phosphotransferase
ATP + pyruvate = ADP + phosphoenolpyruvate
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Thermostable, allows ATP regeneration
Liquid, 50 % (v/v) glycerin
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM ADP
6 mM phosphoenolpyruvate
0.04 g/l PK02 enzyme
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.