The adenylate kinase NMPK23 corresponds to the human gene AK9. It catalyzes the phosphorylation of AMP, dAMP, CMP and dCMP with ATP as phosphate donor and of AMP and CMP with GTP as phosphate donor. Next to its function as nucleoside monophosphate kinase it also exhibits nucleoside diphosphate kinase activities on the substrates (d)CDP, UDP, (d)ADP, (d)GDP and dTTP with either ATP or GTP as phosphate donor.
hAK9, AMPK, 5'-AMP-kinase, adenylic kinase, adenylokinase, adenylate kinase isoenzyme 9, adenylate kinase domain-containing protein 1, AKD1, C6orf199, myokinase, ATP:AMP phosphotransferase
ATP + AMP = 2 ADP
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Mesophilic, additional NDPK activity
Liquid, 50 % (v/v) glycerin
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM AMP
6 mM ATP
0.04 g/l NMPK23 enzyme
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.