The prokaryotic UMP kinase NMPK05 is specific for UMP as substrate and is used by prokaryotes in the de novo synthesis of pyrimidines, in contrast to eukaryotes, which use the dual-specificity enzyme UMP/CMP kinase (EC 188.8.131.52) for the same purpose. Secondary substrates are GMP and - to a much lesser extent - CMP. This enzyme is the subject of feedback regulation, being inhibited by UTP and activated by GTP. NMPK05 is thermostable up to 80°C.
UMPK, uridylate kinase, uridine monophosphate kinase, ATP:UMP phosphotransferase
ATP + UMP = ADP + UDP
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Thermostable up to 80°C
Liquid, 50 % (v/v) glycerin
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM UMP
6 mM ATP
0.04 g/l NMPK05 enzyme
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.