The prokaryotic guanylate kinase NMPK03 catalyzes the phosphorylation of GMP, dGMP, AMP and, to a small extent, dAMP. ATP and dATP can act as donor. NMPK03 is thermostable up to 80°C.
GMPK, deoxyguanylate kinase, guanosine monophosphate kinase, 5'-GMP kinase, ATP:GMP phosphotransferase, ATP:(d)GMP phosphotransferase
ATP + GMP = ADP + GDP
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Thermostable up to 80°C
Liquid, 50 % (v/v) glycerin
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM GMP
6 mM ATP
0.04 g/l NMPK03 enzyme
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.