The thymidine kinase NK16 is a mesophilic enzyme of viral origin. The enzyme phosphorylates thymidine, uridine, deoxyuridine, deoxycytidine and deoxyguanosine as well as a number of modified pyrimidine and purine derivatives. ATP or dGTP can act as phosphate donor. The quasi-irreversible transfer of phosphate from the high energy phosphate donor to the nucleoside enables high NMP yields.
TK, 2'-deoxythymidine kinase, ATP:thymidine 5'-phosphotransferase
ATP + thymidine = ADP + dTMP
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Liquid, 50 % (v/v) glycerin
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM thymidine
6 mM ATP
0.04 g/l NK16 enzyme
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.