The adenosine kinase NK14 originates from the human ADK gene. It catalyzes the phosphorylation of the purine nucleoside adenosine to AMP and also accepts deoxyadenosine and other related nucleoside analogs as substrates. The quasi-irreversible transfer of phosphate from the high energy phosphate donor to the nucleoside enables high NMP yields.
hADK, AK, ATP:adenosine 5'-phosphotransferase
ATP + adenosine = ADP + AMP
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Specific for adenosine-related substrates
Liquid, 50 % (v/v) glycerin
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM adenosine
6 mM ATP
0.04 g/l NK14 enzyme
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.