Thermostable enzyme of bacterial origin. Phosphorylates thymidine and its derivatives to the respective NMPs using ATP as phosphate donor. Deoxycytidine, deoxyguanosine, deoxyinosine, uridine and deoxyuridine can also act as acceptor, dGTP can act as a donor. The quasi-irreversible transfer of phosphate from the high energy donor to the nucleoside enables high NMP yields.
TK, 2'-deoxythymidine kinase, ATP:thymidine 5'-phosphotransferase
ATP + thymidine = ADP + dTMP
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Thermostable up to 50°C
Liquid, 50 % (v/v) glycerin
Example reaction conditions
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.