The glucose-6-phosphate dehydrogenase DH01 is of bacterial origin and thermostable up to 80°C. The G6PDH reduces NADP+ to NADPH while oxidizing D-glucose-6-phosphate to 6-phospho-D-glucono-1,5-lactone as part of the pentose phosphate pathway.
G6PDH, 6-phosphoglucose dehydrogenase, D-glucose 6-phosphate dehydrogenase, Entner-Doudoroff enzyme, G6PD, GPD, NADP-dependent glucose 6-phosphate dehydrogenase, D-glucose-6-phosphate:NADP+ 1-oxidoreductase
D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Thermostable up to 80°C
Liquid, 50 % (v/v) glycerin
Example reaction conditions
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.