Adenylate kinase NMPK23
The adenylate kinase NMPK23 corresponds to the human gene AK9. It catalyzes the phosphorylation of AMP, dAMP, CMP and dCMP with ATP as phosphate donor and of AMP and CMP with GTP as phosphate donor. Next to its function as nucleoside monophosphate kinase it also exhibits nucleoside diphosphate kinase activities on the substrates (d)CDP, UDP, (d)ADP, (d)GDP and dTTP with either ATP or GTP as phosphate donor.
E-KI-0223
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Product Information
EC Number
2.7.4.3
CAS Number
9013-02-9
Synonyms
hAK9, AMPK, 5'-AMP-kinase, adenylic kinase, adenylokinase, adenylate kinase isoenzyme 9, adenylate kinase domain-containing protein 1, AKD1, C6orf199, myokinase, ATP:AMP phosphotransferase
Catalyzed reaction(s)
ATP + AMP = 2 ADP
Properties
Origin
Homo sapiens
Molecular weight
49.8 kDa
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Mesophilic, additional NDPK activity
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM AMP
6 mM ATP
0.04 g/l NMPK23 enzyme
37°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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